HRC Polyclonal antibody, PBS Only

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• HRC Polyclonal antibody, PBS Only (18142-1-PBS)

• 

  WB analysis of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 18142-1-AP (same clone as 18142-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 18142-1-AP (same clone as 18142-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 18142-1-AP (same clone as 18142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18142-1-AP (same clone as 18142-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18142-1-AP (HRC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18142-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

HRC Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 18142-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

18142-1-PBS targets HRC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12794

  Product name: Recombinant human HRC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 26-350 aa of BC069795

  Sequence: MTQQLRGDGLGFRNRNNSTGVAGLSEEASAELRHHLHSPRDHPDENKDVSTENGHHFWSHPDREKEDEDVSKEYGHLLPGHRSQDHKVGDEGVSGEEVFAEHGGQARGHRGHGSEDTEDSAEHRHHLPSHRSHSHQDEDEDEVVSSEHHHHILRHGHRGHDGEDDEGEEEEEEEEEEEEASTEYGHQAHRHRGHGSEEDEDVSDGHHHHGPSHRHQGHEEDDDDDDDDDDDDDDDDVSIEYRHQAHRHQGHGIEEDEDVSDGHHHRDPSHRHRSHEEDDNDDDDVSTEYGHQAHRHQDHRKEEVEAVSGEHHHHVPDHRHQGHRD

• Full Name: histidine rich calcium binding protein
• Calculated Molecular Weight: 699 aa, 80 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC069795
• Gene Symbol: HRC
• Gene ID (NCBI): 3270
• RRID: AB_10598013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23327
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.