HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L)

Applications

ELISA, WB, Dot blot

Conjugate

HRP

Host

Goat

Reactivity

Rabbit

Publications

8100

Reviews (6)

Cat no : SA00001-2

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Validation Data Gallery

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  IP Result of anti-alpha Tubulin (IP: 66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug. SA00001-2 (HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) ) as the secondary antibody.

Product Information

• Concentration: 0.2 mg/mL
• Applications: ELISA, WB, Dot blot
• Host Species: Goat
• Species Reactivity: Rabbit
• Physical State: Liquid
• Suggested Dilution Range: 1:2000-1:10,000 for western blotting with ECL substrates; 1:1000-1:20,000 for ELISA and Western blotting with chromogenic substrates
• Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
• Storage Buffer: 0.01 M Sodium phosphate, 0.25 M NaCl, 50% glycerol, 3 mg/ml BSA, pH 7.6.
• Storage Instructions: Store at -20°C. Stable for one year after shipment.
• Stabilizer: 3 mg/ml BSA
• Clonality: Polyclonal
• RRID: AB_2722564
• Note: This product is for research use only,not for diagnostic or therapeutic use.

Publications

Title
Nature; Structural basis for FGF hormone signalling; Authors - Lingfeng Chen
Nat Biotechnol; Generation of a live attenuated influenza A vaccine by proteolysis targeting.; Authors - Longlong Si
Nat Methods; Metabolomic profiling of single enlarged lysosomes.; Authors - Hongying Zhu
Cell Host Microbe; Bifidobacterium longum promotes postoperative liver function recovery in patients with hepatocellular carcinoma; Authors - Jingjing Yu
Nat Hum Behav; Genome-wide meta-analysis, functional genomics and integrative analyses implicate new risk genes and therapeutic targets for anxiety disorders; Authors - Wenqiang Li
Nat Genet; Comprehensive characterization of protein-protein interactions perturbed by disease mutations.; Authors - Feixiong Cheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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• FHMacarena (Verified Customer) (10-07-2022):

  good results when we are running out of our secondaries of choice

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: cell lines
• FHSONIA (Verified Customer) (06-13-2022):
  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Protein samples from tumor cell lines
• FHBrogan (Verified Customer) (02-22-2022):

  Used for an ELISA against a custom made polyclonal antibody and worked brilliantly. Will definitely be ordering this secondary antibody again.

  • Applications: Other
• FHArturo (Verified Customer) (02-17-2022):

  Amazing product! I manage to reproduce the same results using the sample now that I was running out of my second antibody for the Western Blots. Now I can take care of the rest of my experiments and finish them for my PhD project.

  • Applications: Western Blot
• FHPaolo (Verified Customer) (03-05-2020):

  it works perfectly in ELISA experiments

  • Applications: Other,
• FHDavid (Verified Customer) (06-19-2019):

  I use this or an anti-mouse HRP conjugate about everyday, I bought this one after trying the mouse version that was previously here in the lab. I like it very much. Most of the time I get excellent and sharp results. The times I don't I think it's more the experiment than your product.

  • Applications: Western Blot,

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