HSD11B2 Polyclonal antibody

HSD11B2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 14192-1-AP

Synonyms

11-beta-hydroxysteroid dehydrogenase type II, 11-beta-hydroxysteroid dehydrogenase type 2, 11-beta-HSD2, 11-beta-HSD type II, 11 DH2

---

Validation Data Gallery

• 

  WB analysis using 14192-1-AP

  Human placenta tissue lysates were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 14192-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 14192-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14192-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14192-1-AP (HSD11B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 14192-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14192-1-AP (HSD11B2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14192-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14192-1-AP (HSD11B2 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: human placenta tissue, mouse kidney tissue, human kidney tissue, Transfected HEK-293 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Product Information

14192-1-AP targets HSD11B2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSD11B2 fusion protein Ag5146
• Full Name: hydroxysteroid (11-beta) dehydrogenase 2
• Calculated Molecular Weight: 405 aa, 44 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC036780
• Gene Symbol: HSD11B2
• Gene ID (NCBI): 3291
• RRID: AB_2119643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HSD11B2 gene encodes the type II isoform of 11-beta-hydroxysteroid dehydrogenase, a microsomal enzyme complex responsible for the interconversion of biologically active cortisol and inactive cortisone. This protein catalyzes the cortisol-to-cortisone reaction. This protein expresses approximately 44 kDa in placenta, trophoblast, and distal colon, but in kidney tissue, there are two bands of approximately 44 and 48 kDa can be consistently observed and no signal is seen in decidua, adrenal, or liver(PMID:9048640). HSD11B2 protein was detected in CEM-C7 cells via immunoblot analysis as a dimer and trimer of approximately 80 and 120 kDa (PMID:23762608).

Publications

Species	Application	Title
rat	WB	Nutrients; Maternal High-Fructose Corn Syrup Intake Impairs Corticosterone Clearance by Reducing Renal 11β-Hsd2 Activity via miR-27a-Mediated Mechanism in Rat Offspring; Authors - Yuki Nouchi
mouse	WB	Int Immunopharmacol; Mineralocorticoid promotes intestinal inflammation through receptor dependent IL17 production in ILC3s; Authors - Rongchuan Zhao
rat	WB	Oncotarget; Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.; Authors - Shuai Lian
mouse	WB	Ecotoxicol Environ Saf; Heat stress affects fetal brain and intestinal function associated with the alterations of placental barrier in late pregnant mouse.; Authors - Huiduo Guo
human	WB,IHC	Carcinogenesis; Modulation of the oral glucocorticoid system during black raspberry mediated oral cancer chemoprevention.; Authors - Divya Nedungadi ; KD Validated
pig	WB	Sci Rep; Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets.; Authors - Haodong Chen