HSD17B6 Monoclonal antibody

HSD17B6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D4G6

Cat no : 67496-1-Ig

Synonyms

17 beta HSD 6, 17 beta HSD6, 3 alpha >beta HSE, HSD17B6, HSE, RODH, SDR9C6

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Validation Data Gallery

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  WB analysis using 67496-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67496-1-Ig (HSD17B6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 67496-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 67496-1-Ig (HSD17B6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67496-1-Ig (HSD17B6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67496-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67496-1-Ig (HSD17B6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: JAR cells, Daudi cells, HuH-7 cells, HepG2 cells, L02 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:100-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67496-1-Ig targets HSD17B6 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HSD17B6 fusion protein Ag17330
• Full Name: hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse)
• Calculated Molecular Weight: 317 aa, 36 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC020710
• Gene Symbol: HSD17B6
• Gene ID (NCBI): 8630
• RRID: AB_2882720
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.