HSL Polyclonal antibody, PBS Only

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• HSL Polyclonal antibody, PBS Only (17333-1-PBS)

• 

  WB analysis using 17333-1-AP (same clone as 17333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17333-1-AP (HSL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brown adipose using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17333-1-AP (same clone as 17333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17333-1-AP (HSL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17333-1-PBS in a different storage buffer formulation.

HSL Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 17333-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

LIPE, REH, LHS, Hormone-sensitive lipase, EC:3.1.1.79

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Product Information

17333-1-PBS targets HSL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11360

  Product name: Recombinant human LIPE protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 728-1076 aa of BC070041

  Sequence: GNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH

• Full Name: lipase, hormone-sensitive
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC070041
• Gene Symbol: HSL
• Gene ID (NCBI): 3991
• RRID: AB_2878386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05469
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.