HSPB2 Polyclonal antibody

HSPB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21755-1-AP

Synonyms

Heat shock protein family B member 2, Heat shock protein beta-2, Heat shock protein beta 2, heat shock 27kDa protein 2, DMPK-binding protein

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 21755-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21755-1-AP (HSPB2 antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

• 

  WB analysis of human skeletal muscle using 21755-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21755-1-AP (HSPB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 21755-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 21755-1-AP (HSPB2 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 21755-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 21755-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 21755-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21755-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21755-1-AP

  Immunofluorescent analysis of HepG2 cells, using HSPB2 antibody 21755-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, rat heart tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

21755-1-AP targets HSPB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSPB2 fusion protein Ag16146
• Full Name: heat shock 27kDa protein 2
• Calculated Molecular Weight: 182 aa, 20 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC109393
• Gene Symbol: HSPB2
• Gene ID (NCBI): 3316
• RRID: AB_10859789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cancer; miR-17-5p promotes the invasion and migration of colorectal cancer by regulating HSPB2.; Authors - Weifang Yu
human	WB,IHC	Clin Epigenetics; PMID: 31088577; Authors - An-An Yin