HSPB2 Polyclonal antibody

HSPB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21755-1-AP

Synonyms

Heat shock protein family B member 2, Heat shock protein beta-2, Heat shock protein beta 2, heat shock 27kDa protein 2, DMPK-binding protein



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, rat heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21755-1-AP targets HSPB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPB2 fusion protein Ag16146
Full Name heat shock 27kDa protein 2
Calculated Molecular Weight 182 aa, 20 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC109393
Gene Symbol HSPB2
Gene ID (NCBI) 3316
RRIDAB_10859789
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HSPB2 antibody 21755-1-APDownload protocol
IHC protocol for HSPB2 antibody 21755-1-APDownload protocol
IF protocol for HSPB2 antibody 21755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cancer

miR-17-5p promotes the invasion and migration of colorectal cancer by regulating HSPB2.

Authors - Weifang Yu
humanWB,IHC

Clin Epigenetics

PMID: 31088577

Authors - An-An Yin