HSP90AB1 Recombinant antibody

HSP90AB1 Recombinant Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5P14

Cat no : 80301-1-RR

Synonyms

D6S182, FLJ26984, Heat shock 84 kDa, Heat shock protein HSP 90 beta, HS90B, HSP 84, HSP 90, HSP84, HSP90 BETA, HSP90AB1, HSP90B, HSPC2, HSPCB



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, Neuro-2a cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

80301-1-RR targets HSP90AB1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HSP90AB1 fusion protein Ag1947
Full Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Calculated molecular weight 723 aa, 83 kDa
GenBank accession numberBC014485
Gene symbol HSP90AB1
Gene ID (NCBI) 3326
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers.

Protocols

Product Specific Protocols
WB protocol for HSP90AB1 antibody 80301-1-RRDownload protocol
IHC protocol for HSP90AB1 antibody 80301-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols