HSP90 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of zebrafish using 80301-1-RR (same clone as 80301-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80301-1-RR (HSP90AB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80301-1-RR (same clone as 80301-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80301-1-RR (HSP90AB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 80301-1-RR (same clone as 80301-1-PBS)

  WB result of HSP90AB1 antibody (80301-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells. This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 80301-1-RR (same clone as 80301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80301-1-RR (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 80301-1-RR (same clone as 80301-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP90AB1 antibody (80301-1-RR, Clone: 5P14 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80301-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80301-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80301-1-RR against Human HSP90AB1 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

HSP90 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 80301-1-PBS

Clone No.5P14

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HSP90AB1, 5P14, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta

---

Product Information

80301-1-PBS targets HSP90 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1947

  Product name: Recombinant human HSP90AB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 330-710 aa of BC014485

  Sequence: RALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPP

• Full Name: heat shock protein 90kDa alpha (cytosolic), class B member 1
• Calculated Molecular Weight: 723 aa, 83 kDa
• Observed Molecular Weight: 83-90 kDa
• GenBank Accession Number: BC014485
• Gene Symbol: HSP90AB1
• Gene ID (NCBI): 3326
• RRID: AB_2918885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08238
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.