HSPA1L Polyclonal antibody

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• HSPA1L Polyclonal antibody (13970-1-AP)

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HSPA1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13970-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Heat shock 70 kDa protein 1-Hom, Heat shock 70 kDa protein 1-like, Heat shock protein family A member 1L, HSP70 1L, HSP70 HOM

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  WB analysis using 13970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat testis using 13970-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 13970-1-AP (HSPA1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 13970-1-AP

  IP result of anti-HSPA1L (IP:13970-1-AP, 4ug; Detection:13970-1-AP 1:1000) with HepG2 cells lysate 1800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human endometrial cancer using 13970-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human endometrial cancer using 13970-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 13970-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13970-1-AP (HSPA1L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 13970-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 13970-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13970-1-AP (HSPA1L antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 13970-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSPA1L antibody (13970-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, rat testis tissue, HepG2 cells, human saliva sample, mouse liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human endometrial cancer tissue, human breast cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13970-1-AP targets HSPA1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4995

  Product name: Recombinant human HSPA1L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 343-641 aa of BC034483

  Sequence: TRIPKVQRLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTALMKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFDLTGIPPAPRGVPQIEVTFDIDANGILNVTAMDKSTGKVNKITIANDKGRLSKEEIERMVLDAEKYKAEDEVQREKIAAKNALESYAFNMKSVVSDEGLKGKISESDKNKILDKCNELLSWLEVNQLAEKDEFDHKRKELEQMCNPIITKLYQGGCTGPACGTGYVPGRPATGPTIEEVD

• Full Name: heat shock 70kDa protein 1-like
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC034483
• Gene Symbol: HSPA1L
• Gene ID (NCBI): 3305
• RRID: AB_2119675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P34931
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognized the endogenous HSPA1L protein in multiple cell lines. (21730052)

Publications

What published studies show

The HSPA1L Polyclonal antibody (Cat# 13970-1-AP) has 8 citations published in Nature, J Cachexia Sarcopenia Muscle, Neurobiology of Disease, Frontiers in Endocrinology, Biomolecules, Virology Journal, and PeerJ. Research fields span epithelial-to-mesenchymal transition, mitophagy in diabetic sarcopenia, ALS pathology, hepatic unfolded protein response, mastitis inflammation and apoptosis, mitochondrial proteomics in avian influenza infection, and heat shock protein roles in male fertility.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC,IF	Nature; Evolutionary fingerprints of epithelial-to-mesenchymal transition; Authors - Luigi Perelli
mouse	WB	J Cachexia Sarcopenia Muscle; The regulation of MFG-E8 on the mitophagy in diabetic sarcopenia via the HSPA1L-Parkin pathway and the effect of D-pinitol; Authors - Wenqian Zhao
mouse	IF	Neurobiol Dis; SBT-272 improves TDP-43 pathology in ALS upper motor neurons by modulating mitochondrial integrity, motility, and function; Authors - Mukesh Gautam
rat	WB	Front Endocrinol (Lausanne); Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring.; Authors - Xiaomei Liu
mouse,bovine	WB	Biomolecules; Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model; Authors - Bo Fang
mouse,bovine	WB	Biomolecules; Targeted Regulation of HSP70 by the ARP2/3 Complex in Mammary Epithelial Cells and Its Impact on Host Cell Apoptosis; Authors - Tingji Yang