HYAL1 Monoclonal antibody

HYAL1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1B8B5

Cat no : 66560-1-Ig

Synonyms

HYAL 1, HYAL1, Hyaluronidase 1, hyaluronoglucosaminidase 1, LuCa 1, LUCA1, Lung carcinoma protein 1, NAT6

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 66560-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66560-1-Ig (HYAL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HepG2 cells, LNCaP cells, PC-3 cells, DU 145 cells, NCCIT cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

66560-1-Ig targets HYAL1 in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HYAL1 fusion protein Ag18183
• Full Name: hyaluronoglucosaminidase 1
• Calculated Molecular Weight: 435 aa, 48 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC035695
• Gene Symbol: HYAL1
• Gene ID (NCBI): 3373
• RRID: AB_2881921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL-1 is present in many tissues and is predominantly found in the plasma and urine (PMID: 16600643). In addition to its function in normal cellular hyaluronan turnover, human HYAL-1 is implicated in cancer proliferation, angiogenesis, and inflammatory diseases (PMID: 17503783).

Publications

Species	Application	Title
human	WB	J Immunother Cancer; An oncolytic vaccinia virus encoding hyaluronidase reshapes the extracellular matrix to enhance cancer chemotherapy and immunotherapy; Authors - Shibing Wang
human,mouse	WB,IF	Appl Biochem Biotechnol; Kunzea Ericoides (Kanuka) Leaf Extracts Show Moisturisation, Antioxidant, and UV Protection Effects in HaCaT Cells and Anti-melanogenesis Effects in B16F10 Cells; Authors - Xuefeng He