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Harmonin Polyclonal antibody, PBS Only (11358-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 11358-1-AP (same clone as 11358-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 11358-1-AP (same clone as 11358-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 11358-1-AP (same clone as 11358-1-PBS)
IP result of anti-Harmonin (IP:11358-1-AP, 4ug; Detection:11358-1-AP 1:600) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.
IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)
Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.
IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)
Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.
IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)
Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.
IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)
Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.
IHC staining of Human Colon cancer using 11358-1-AP (same clone as 11358-1-PBS)
Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 11358-1-AP (same clone as 11358-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Harmonin antibody (11358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11358-1-PBS in a different storage buffer formulation.

Harmonin Polyclonal antibody, PBS Only

Name: Harmonin Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 11358-1-PBS

Harmonin Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11358-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

11358-1-PBS targets Harmonin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1919 Product name: Recombinant human USH1C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-392 aa of BC016057 Sequence: MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPSRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPDEPLTWQYVDQFVSESGGVRGSLGSPGNRENKEKKVFISLVGSRGLGCSISSGPIQKPGIFISHVKPGSLSAEVGLEIGDQIVEVNGVDFSNLDHKEGRELFMTDRERLAEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQIVEEEEKFKKQWEEDWGSKEQLLLPKTITAEVHPVPLRKPKYDQGVEPE Predict reactive species
Full Name	Usher syndrome 1C (autosomal recessive, severe)
Calculated Molecular Weight	552 aa, 62 kDa
Observed Molecular Weight	68-73 kDa
GenBank Accession Number	BC016057
Gene Symbol	USH1C/Harmonin
Gene ID (NCBI)	10083
RRID	AB_2214137
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y6N9
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Harmonin, also known as USH1C, is a scaffold protein which interacts with other USH1 proteins (USH1B/myosin VIIa and USH1G/sans) to form complex and is implicated in proper development, morphogenesis, and structural integrity of the hair cell mechanoreceptor. Mutations of Harmonin cause Usher syndrome type 1 (congenital deafness and balance disorder, and early-onset sight loss).