Mouse HGF Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01760-3

  Sandwich ELISA standard curve of MP01760-3, Mouse HGF Recombinant Matched Antibody Pair - PBS only. 85014-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0675. 85014-3-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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  Sample test of MP01760-3

  The mean HGF concentration was determined to be 2,919.6 pg/mL in mouse kidney tissue extract based on a 3.1 mg/mL extract load and 13,658.8 pg/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load.

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  Sample test of MP01760-3

  Serum of six mice was measured. The HGF concentration of detected samples was determined to be 7,505.4 pg/mL with a range of 5,590.3-9,962.8 pg/mL.

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  Sample test of MP01760-3

  Serum of six rats was measured. The HGF concentration of detected samples was determined to be 40,922.2 pg/mL with a range of 21,345.0-50,183.1 pg/mL.

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  Sample test of MP01760-3

  3T3-L1 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse HGF, and measured 3,491.8 pg/mL.

HGF Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85014-3-PBS

Clone No.242476H8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Hepatocyte growth factor, Hepatocyte growth factor alpha chain, Hepatocyte growth factor beta chain, Hepatopoietin-A, Scatter factor

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Product Information

85014-3-PBS targets HGF as part of a matched antibody pair:

MP01760-3: 85014-4-PBS capture and 85014-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0675

  Product name: Recombinant Mouse Hgf protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 33-728 aa of NM_001289458.1

  Sequence: QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKKVNSADECANRCIRNRGFTFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKTCAHSAVNETDVPMETTECIQGQGEGYRGTSNTIWNGIPCQRWDSQYPHKHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADGLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL

• Full Name: hepatocyte growth factor
• Calculated Molecular Weight: 83KD
• GenBank Accession Number: NM_001289458.1
• Gene Symbol: Hgf
• Gene ID (NCBI): 15234
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q08048-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. HGF is derived from a biologically inactive single chain precursor of 728 amino acids (pro-HGF) localized mostly on the cell surface and in the extracellular matrix. The mature form produced following proteolytic cleavage is composed of a 69-kDa α-subunit (containing four kringle domains) and the 34 kDa β-subunit, similar to the catalytic domain of serine proteases, but with amino acid substitutions in the active site. HGF is a pleiotropic cytokine which exerts a variety of effects on several cells, being involved in the regulation of many biological processes, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities.