Histone H2A.X Recombinant antibody, PBS Only

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  WB analysis using 83307-3-RR (same clone as 83307-3-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 83307-3-RR (Histone H2A.X antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83307-3-PBS in a different storage buffer formulation.

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  IHC staining of human kidney(renal cell) Cancer using 83307-3-RR (same clone as 83307-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney(renal cell) Cancer slide using 83307-3-RR (Histone H2A.X antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83307-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney(renal cell) Cancer using 83307-3-RR (same clone as 83307-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney(renal cell) Cancer slide using 83307-3-RR (Histone H2A.X antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83307-3-PBS in a different storage buffer formulation.

Histone H2A.X Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 83307-3-PBS

Clone No.250032F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

H2AFX, H2A histone family, member X, H2A.X, H2AX, Histone H2A

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Product Information

83307-3-PBS targets Histone H2A.X in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Histone H2A.X fusion protein Ag1305
• Full Name: H2A histone family, member X
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15-18 kDa, 22 kDa
• GenBank Accession Number: BC013416
• Gene Symbol: Histone H2A.X
• Gene ID (NCBI): 3014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16104
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.