Histone H2A.X Recombinant antibody

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  WB analysis using 83307-3-RR

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 83307-3-RR (Histone H2A.X antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney(renal cell) Cancer using 83307-3-RR

  Immunohistochemical analysis of paraffin-embedded human kidney(renal cell) Cancer slide using 83307-3-RR (Histone H2A.X antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney(renal cell) Cancer using 83307-3-RR

  Immunohistochemical analysis of paraffin-embedded human kidney(renal cell) Cancer slide using 83307-3-RR (Histone H2A.X antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Histone H2A.X Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 83307-3-RR

Clone No.250032F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

H2AFX, H2A histone family, member X, H2A.X, H2AX, Histone H2A

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Tested Applications

• Positive WB detected in: HEK-293T cells, NIH/3T3 cells, C6 cells
• Positive IHC detected in: human kidney(renal cell) Cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83307-3-RR targets Histone H2A.X in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Histone H2A.X fusion protein Ag1305
• Full Name: H2A histone family, member X
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15-18 kDa, 22 kDa
• GenBank Accession Number: BC013416
• Gene Symbol: Histone H2A.X
• Gene ID (NCBI): 3014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16104
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.