Histone H2A.X Recombinant antibody

Histone H2A.X Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83307-3-RR
Clone No.250032F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

H2AFX, H2A histone family, member X, H2A.X, H2AX, Histone H2A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHEK-293T cells, NIH/3T3 cells, C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83307-3-RR targets Histone H2A.X in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Histone H2A.X fusion protein Ag1305 Predict reactive species
Full Name H2A histone family, member X
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15-18 kDa, 22 kDa
GenBank Accession NumberBC013416
Gene Symbol Histone H2A.X
Gene ID (NCBI) 3014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16104
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.

Protocols

Product Specific Protocols
WB protocol for Histone H2A.X antibody 83307-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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