Histone H2B Monoclonal antibody

Histone H2B Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, Mouse, Rat, Zebrafish, Chicken and More (1)







Cat no : 68393-1-Ig


GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, chicken cerebellum tissue, zebrafish tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68393-1-Ig targets Histone H2B in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat, Zebrafish, Chicken samples.

Tested Reactivity Human, Mouse, Rat, Zebrafish, Chicken
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name histone cluster 2, H2be
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC005827
Gene Symbol HIST2H2BE
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).


Product Specific Protocols
WB protocol for Histone H2B antibody 68393-1-IgDownload protocol
IHC protocol for Histone H2B antibody 68393-1-IgDownload protocol
IF protocol for Histone H2B antibody 68393-1-IgDownload protocol
FC protocol for Histone H2B antibody 68393-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Clin Epigenetics

Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis

Authors - Xiangjun Yin