CoraLite® Plus 488-conjugated Histone H2B Monoclonal antibody

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  IF Staining of HeLa using CL488-68393

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Histone H2B antibody (CL488-68393, Clone: 1E12G3 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-68393

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Histone H2B Monoclonal antibody (CL488-68393, Clone:1E12G3)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Histone H2B Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-68393

Clone No.1E12G3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, chicken, zebrafish

Applications

IF/ICC, FC (Intra)

Synonyms

H2B, HIST2H2BE, 1E12G3, GL105, H2B.1

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68393 targets Histone H2B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, chicken, zebrafish samples.

• Tested Reactivity: human, mouse, rat, chicken, zebrafish
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 2, H2be
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14-17 kDa
• GenBank Accession Number: BC005827
• Gene Symbol: Histone H2B
• Gene ID (NCBI): 8349
• RRID: AB_3084480
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q16778
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).