Recombinant Human Pancreatic alpha-amylase protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3071

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Validation Data Gallery

• 

  Purity of Recombinant Human Pancreatic alpha-amylase was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Pancreatic alpha-amylase protein Gln16-Leu511 (Accession# P04746-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 279
• Accession: P04746-1
• PredictedSize: 81.9 kDa
• SDS-PAGE: 75-90 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

AMY2A, or amylase alpha 2A, is a gene in humans that encodes for a specific isoenzyme of the alpha-amylase family of proteins. AMY2A is expressed at high levels in the pancreas and is selectively found in the cytoplasm of pancreatic exocrine glandular cells. This protein is a secreted enzyme that plays a crucial role in the digestive process, particularly in the breakdown of dietary starch and glycogen.

References:

1. Nakajima K. (2016). World J Diabetes. 25;7(6):112-21. 
2. Hariharan R. et al. (2021). Obes Rev. 22(6):e13205.