Recombinant Human Angiopoietin 4 protein (rFc Tag)

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• Recombinant Human Angiopoietin 4 protein (rFc Tag) (Eg4718)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4718

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Validation Data Gallery

• 

  Purity of Recombinant Human Angiopoietin 4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Angiopoietin 4 protein Met282-Ile503 (Accession# Q9Y264-1) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 51378
• Accession: Q9Y264-1
• PredictedSize: 52.4 kDa
• SDS-PAGE: 55-70 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

As one of the factors regulating tumour angiogenesis, ANGPT4 (angiopoietin-4), which plays an important role in promoting tumour proliferation, survival, expansion and angiogenesis, is highly expressed in some tumours, such as lung adenocarcinoma, glioblastoma and ovarian cancer. In addition, other studies have shown that the expression of ANGPT4 in tumours may regulate the occurrence and development of tumours by affecting the PI3K-Akt signalling pathway, the Hedgehog signalling pathway.

References:

1. Wenchao Zhou. et al. (2024). Clin Exp Med. 24(1):224.
2. Alba Matas-Céspedes. et al. (2014). Clin Cancer Res. 20(13):3458-3471.
3. A Shaw. et al. (2009). Oncogene. 28(50):4480-4490.