Recombinant Human Angiogenin protein (mFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

mFc Tag

Activity

not tested

Cat no : Eg1931



Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity
Not tested
Expression HEK293-derived Human Angiogenin protein Gln25-Pro147 (Accession# P03950) with a mouse IgG Fc tag at the N-terminus.
GeneID 283
Accession P03950
PredictedSize 41.8 kDa
SDS-PAGE 39-45 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC.

References:

1. Miyake M. et al.(2015). Oncogene. 34(7):890-901. 2. Hartmann A. et al. (1999). Cancer Res. 59(7):1578-83. 3. Kishimoto K. et al. (2005). Oncogene. 13;24(3):445-56. 4. Bárcena C. et al.(2015). Sci Rep. 5:7916.