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Recombinant Human Apolipoprotein A II protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3085

Validation Data Gallery

  • Purity of Recombinant Human Apolipoprotein A II was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Apolipoprotein A II was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human Apolipoprotein A II protein Ala19-Gln100 (Accession# P02652) with a rabbit IgG Fc tag at the C-terminus.
GeneID 336
Accession P02652
PredictedSize 35.3 kDa
SDS-PAGE 35-42 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Apolipoprotein A2 (Apo-A2) is the second major protein of the HDL-C particles and comprises about 20% of the total HDL-C protein content. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer.

References:

1.Bandarian F. et al. (2016) Iranian biomedical journal. 20(2), 84-90.
2.Moradi M. et al. (2017) Clinical Nutrition. 30, 1e5.
3.Ding, X. et al. (2018) Exp anim tokyo. 67(2):105-115.
4.Kobayashi, T. et al. (2018) Biochem bioph res co. 497(3):903-907.