Recombinant Human B7-H6 protein (rFc Tag) (HPLC verified)

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• Recombinant Human B7-H6 protein (rFc Tag) (HPLC verified) (Eg2568)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2568

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Validation Data Gallery

• 

  Purity of Recombinant Human B7-H6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human B7-H6 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human B7-H6 protein Asp25-Ser262 (Accession# Q68D85) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 374383
• Accession: Q68D85
• PredictedSize: 53.0 kDa
• SDS-PAGE: 50-70 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

B7-H6, also known as NCR3LG1, is a member of the B7 family of immunoreceptors. It is a 454-aa-long type I transmembrane protein consisting of an extracellular region, a transmembrane segment, and a cytoplasmic domain which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), an SH2-binding domain, and an SH3-binding motif. B7-H6 triggers NKp30-mediated activation of human NK cells. It has been reported that B7H6 is expressed in various types of tumor cells and plays a prominent role in natural killer cell immunosurveillance. The apparent molecular weight of B7-H6 is larger than the calculated molecular weight of 51 kDa due to glycosylation.

References:

1. Cameron S Brandt. et al. (2009). J Exp Med. 206(7):1495-503.
2. Alaleh Mohammadi. et al. (2022). Life Sci.304:120709. 
3. Ying Chen. et al. (2018). Pathol Oncol Res.4(4):717-721.