Recombinant Human BACE1 protein (rFc Tag)

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• Recombinant Human BACE1 protein (rFc Tag) (Eg4497)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4497

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Validation Data Gallery

• 

  Purity of Recombinant Human BACE1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human BACE1 protein Thr22-Thr457 (Accession# P56817-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 23621
• Accession: P56817-1
• PredictedSize: 74.7 kDa
• SDS-PAGE: 85-95 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

BACE1 is a gene that encodes for an enzyme, also known as Beta-secretase 1. It belongs to the protein class of enzymes and transporters and is predicted to be located in the membrane and intracellular regions. It has several splice variants with different lengths and masses, ranging from 267 to 501 amino acids in length. BACE1 is responsible for the proteolytic processing of the amyloid precursor protein (APP). It cleaves at the N-terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment. This process is a key step in the formation of amyloid beta peptide from amyloid precursor protein, which is central to the development of Alzheimer's disease.

References:

1.Araki W. (2016) Brain Res Bull. 126(Pt 2):170-177.
2.Motoki K. et al. (2012) Brain Behav. 2(3):270-82.
3.Griffiths HH. et al. (2011) J Biol Chem. 286(38):33489-500.