Recombinant Human C1QA protein (rFc Tag)

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• Recombinant Human C1QA protein (rFc Tag) (Eg2396)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2396

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Validation Data Gallery

• 

  Purity of Recombinant Human C1QA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human C1QA protein Glu23-Ala245 (Accession# P02745) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 712
• Accession: P02745
• PredictedSize: 50.9 kDa
• SDS-PAGE: 52-65 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis.

References:

1.Degn, Søren E et al. American journal of human genetics vol. 88,6 (2011): 689-705. 
2.Reid, K B. The Biochemical journal vol. 141,1 (1974): 189-203. 
3.Ling, Guang Sheng et al. Science (New York, N.Y.) vol. 360,6388 (2018): 558-563.