Recombinant Human C4BPB protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2976

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Validation Data Gallery

• 

  Purity of Recombinant Human C4BPB was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human C4BPB protein Ser18-Leu252 (Accession# P20851-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 725
• Accession: P20851-1
• PredictedSize: 52.4 kDa
• SDS-PAGE: 57-80 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Complement 4 binding protein beta chain (C4BPB) is one of the two polypeptides that in humans compose plasma glycoprotein C4b-binding protein (C4BP). C4BPB binds anticoagulant vitamin K-dependent S. C4BPB controls the classical pathway of complement activation. It binds as a cofactor to the C3b/C4b inactivator (C3bINA), hydrolyzing the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a.

References:

1.Rodríguez de Córdoba S. et al. (1994) Genomics. 21(3):501-509.
2.Kadavá T. et al. (2024) EMBO J. 43(14):3009-3026.