Recombinant Human CD14 protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0498

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Validation Data Gallery

• 

  Purity of Recombinant Human CD14 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human CD14 protein Thr20-Met344 (Accession# P08571) with a His tag at the C-terminus.
• GeneID: 929
• Accession: P08571
• PredictedSize: 39.1 kDa
• SDS-PAGE: 45-60 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes. CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS). It plays a major role in the inflammatory response of monocytes to LPS.

References:

1. A Haziot, et al. (1988) J Immunol. 141(2):547-52.
2. D L Simmons, et al. (1989) Blood. 73(1):284-9.
3. S D Wright, et al. (1990) Science. 249(4975):1431-3.
4. A Haziot, et al. (1993) J Immunol. 150(12):5556-65.