Recombinant Human CD30 Ligand/TNFSF8 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2118

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Validation Data Gallery

• 

  Purity of Recombinant Human CD30 Ligand was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human CD30 Ligand protein Gln63-Asp234 (Accession# P32971) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 944
• Accession: P32971
• PredictedSize: 46.8 kDa
• SDS-PAGE: 48-68 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

TNFSF8, also named as CD30LG and CD153, is a member of the tumor necrosis factor (TNF) receptor superfamily. It is the cytokine that binds to TNFRSF8/CD30. It induces proliferation of T-cells. CD30L enhanced the proliferation of CD3-activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines.

References:

1.Ezogelin Oflazoglu, et al. (2009) Adv Exp Med Biol. 647:174-85. 
2.Nancy D Marín, et al. (2017) Tuberculosis (Edinb). 102:8-15. 
3.Jin Duan, et al. (2019) Int Immunopharmacol. 71:350-360.