Speedy™ Human CD4 One-Step ELISA Kit
Cat no : SE50064
Synonyms
CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 4.9 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 920 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 96% | 91%-101% |
| Cell lysate | 89% | 84%-94% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,126.8 | 37.2 | 3.3 |
| 2 | 8 | 273.1 | 2.9 | 1.1 |
| 3 | 8 | 139.4 | 3.4 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,154.8 | 66.1 | 5.7 |
| 2 | 16 | 273.9 | 2.9 | 1.1 |
| 3 | 16 | 140.2 | 4.0 | 2.9 |
Background Information
CD4 is a transmembrane glycoproteinof the immunoglobulin super-family (IgSF), containing four extracellular Ig-like domains, ahydrophobic transmembrane region and a 40-residue cytoplasmictail. CD4 is expressed T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells.CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interactionand it is involved in T cell differentiation, development and activation by interacting with antigen-presenting cells (APCs). The cytoplasmic tail of CD4 associates with the Lck kinase, which in turn activates the signaling components of the T cell receptor (TCR)-CD3 complexes.CD4 also serves as a receptor for human immunodeficiency virus (HIV) by binding to the viral envelope glycoprotein gp120.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.