Recombinant Human CD80/B7-1 protein (C-rFc)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

C-rFc

Activity

not tested

Cat no : Eg3505



Product Information

Purity >90 %, SDS-PAGE
Endotoxin <1.0 EU/μg protein, LAL method
Activity
Not tested
Expression HEK293-derived Human CD80/B7-1 protein Val35-Asn242 (Accession# P33681-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 941
Accession P33681-1
PredictedSize 50.1 kDa
SDS-PAGE
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B.

References:

1. Peach, R J et al. The Journal of biological chemistry vol. 270,36 (1995): 21181-7. 2. Vasilevko, Vitaly et al. DNA and cell biology vol. 21,3 (2002): 137-49. 3. Short, Joshua J et al. Virus research vol. 122,1-2 (2006): 144-53.