Recombinant Human CD81 protein (hFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

hFc Tag

Activity

not tested

Cat no : Eg1035

Overview

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Validation Data Gallery

Purity of Recombinant Human CD81 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Cat No. Eg1035

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Product Information

Purity	>90 %, SDS-PAGE
Endotoxin	<0.1 EU/μg protein, LAL method
Activity	Not tested
Expression	HEK293-derived Human CD81 protein Phe113-Lys201 (Accession# P60033) with a human IgG1 Fc tag at the C-terminus.
GeneID	975
Accession	P60033
PredictedSize	36.5 kDa
SDS-PAGE	34-37 kDa, reducing (R) conditions
Formulation	Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution	Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions	It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Until expiry date, -20℃ to -80℃ as lyophilized proteins. 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125).

References:

1.Oren R.et al.(1990) Mol Cell Biol.10(8):4007-15.
2.Takahashi S.et al.(1990) J Immunol.145(7):2207-13.
3.Kitadokoro K.et al.(2001) EMBO J.20(1-2):12-8.
4.Hemler ME.et al.(2005) Nat Rev Mol Cell Biol.6(10):801-11.
5.Imai T.et al.(1995)J Immunol.155(3):1229-39.