Recombinant Human hCG Alpha protein (rFc Tag) (HPLC verified)

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• Recombinant Human hCG Alpha protein (rFc Tag) (HPLC verified) (Eg3377)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3377

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Validation Data Gallery

• 

  Purity of Recombinant Human hCG Alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human hCG Alpha was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human hCG Alpha protein Ala25-Ser116 (Accession# P01215) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 1081
• Accession: P01215
• PredictedSize: 36.5 kDa
• SDS-PAGE: 38-50 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The glycoprotein hormone human chorionic gonadotropin (hCG) is known for its pregnancy-supporting role. It is produced mainly by trophoblast cells and also produced by the secretory endometrium at the time of implantation. The hCG protein complex is composed of two noncovalently linked subunits, alpha (CGA) and beta (CGB). Both subunits are highly glycosylated. Its alpha subunit is common to luteinizing hormone (LH), follicle-stimulating hormone (FSH) and thyrotropin (TSH), whereas the respective beta-subunits confer the biological specificities.

References:

1.Licht, P. et al. (2007) Mol.Cell.Endocrinol.269,85-92.
2.Tsampalas,M.(2010) J.Reprod.Immunol.85,93-98.
3. Hearn,M.T.(2000) J.Mol.Recognit.13,223-278.