Recombinant Human CIB1 protein (rFc Tag)

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• Recombinant Human CIB1 protein (rFc Tag) (Eg5476)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg5476

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Validation Data Gallery

• 

  Purity of Recombinant Human CIB1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human CIB1 protein Gly2-Leu191 (Accession# Q99828-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 10519
• Accession: Q99828-1
• PredictedSize: 47.2 kDa
• SDS-PAGE: 43-50 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CIB1(Calcium and integrin-binding protein 1) is also named as CIB, KIP, PRKDCIP. It is an EF-hand-containing protein that binds multiple effector proteins, including the platelet αIIbβ3 integrin and several serine/threonine kinases and potentially modulates their function. It regulates platelet aggregation in hemostasis through a specific interaction with the alphaIIb cytoplasmic domain of platelet integrin alphaIIbbeta3.

References:

1. Leisner, Tina M., et al. The FASEB Journal 30.8 (2016): 2640.
2. Wang, Xianwang, et al. Cellular Physiology and Biochemistry 43.4 (2017): 1413-1424.
3. Heineke, Joerg, et al. Nature medicine 16.8 (2010): 872-879.
4. Yoon, Kyoung Wan, et al. Proceedings of the National Academy of Sciences 106.41 (2009): 17389-17394.
5. Zayed, Mohamed A., et al. Circulation research 101.11 (2007): 1185-1193.