Recombinant Human G-CSFR protein (mFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

mFc Tag

Activity

not tested

Cat no : Eg3882

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Validation Data Gallery

• 

  Purity of Recombinant Human G-CSFR was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human G-CSFR was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human G-CSFR protein Glu25-Pro621 (Accession# Q99062-1) with a mouse IgG Fc tag at the C-terminus.
• GeneID: 1441
• Accession: Q99062-1
• PredictedSize: 93.2 kDa
• SDS-PAGE: 95-130 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CSF3R, also known as G-CSFR, is a transmembrane protein called granulocyte colony-stimulating factor receptor, which provides proliferative signals for granulocytes and plays a crucial role in their proliferation and differentiation. CSF3R may also play an important regulatory role in solid tumors.

References:

1. Minglei Huang. et al. (2024). Cent Eur J Immunol. 49(2):155-168.
2. Ioannis Karagiannidis. et al. (2020). Cancers (Basel). 12(10):2868.
3. Ioannis Karagiannidis. et al. (2000). Front Immunol. 11:1885.