Recombinant Human DCC protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2937

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Validation Data Gallery

• 

  Purity of Recombinant Human DCC was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human DCC protein His26-Asn1097 (Accession# P43146) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 1630
• Accession: P43146
• PredictedSize: 144.4 kDa
• SDS-PAGE: 135-180 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Deleted in Colorectal Cancer (DCC) is a single-pass transmembrane protein that belongs to the immunoglobulin superfamily. DCC was originally identified as a prognostic tumor marker and then subsequently found to be a receptor for netrin-1. DCC is expressed in many normal tissues, but was absent in most colorectal carcinomas, hence the name DCC. DCC plays a key role in axon guidance and also in several other important cellular processes.

References:

1.Finci L. et al. (2015) Prog Biophys Mol Biol. 118(3):153-160.
2.Boussouar A. et al. (2020) Cancer Res. 80(4):747-756.
3.Jasmin M. et al. (2021) EMBO J. 40(3):e105537.
4.Lo PS. et al. (2022) J Neurochem. 161(3):254-265.