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Recombinant Human DCIR/CLEC4A protein (rFc Tag)(HPLC verified)

Species

Human

Purity

>95 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg1350

Validation Data Gallery

  • Purity of Recombinant Human DCIR was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human DCIR was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human DCIR/CLEC4A was greater than 90% as determined by SEC-HPLC.

    The purity of Human DCIR/CLEC4A was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >95 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human DCIR protein Gln70-Leu237 (Accession# Q9UMR7-1) with a rabbit IgG Fc tag at the N-terminus.
GeneID 50856
Accession Q9UMR7-1
PredictedSize 47.1 kDa
SDS-PAGE 45-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

C-type lectin domain family 4 member A (CLEC4A) is also known as DCIR, HDCGC13P, CLECSF6, and CD367. CLEC4A is C-type lectin receptor that binds carbohydrates mannose and fucose but also weakly interacts with N-acetylglucosamine (GlcNAc) in a Ca2+-dependent manner. It is involved in regulating immune reactivity. In plasmacytoid dendritic cells, CLEC4A inhibits TLR9-mediated IFNA and TNF production.

References:

1.Nagae M, et al. (2016) FEBS Lett. 590(8):1280-8.
2.Bates EE, et al. (1999) J Immunol. 163(4):1973-83.
3.Meyer-Wentrup F, et al. (2008) Blood. 111(8):4245-53.