Recombinant Human Ephrin B1 protein (mFc Tag) (HPLC verified)

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• Recombinant Human Ephrin B1 protein (mFc Tag) (HPLC verified) (Eg4188)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

mFc Tag

Activity

not tested

Cat no : Eg4188

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Validation Data Gallery

• 

  Purity of Recombinant Human Ephrin B1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human Ephrin B1 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Ephrin B1 protein Leu28-Lys237 (Accession# P98172) with a mouse IgG Fc tag at the C-terminus.
• GeneID: 1947
• Accession: P98172
• PredictedSize: 49.6 kDa
• SDS-PAGE: 52-65 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

EFNB1 (Ephrin B1) is a member of the ephrin family, which are membrane-bound guidance cues that interact with Eph receptors, a family of receptor tyrosine kinases. EFNB1 is X-inactivated, and it has been proposed that in heterozygous females, patchy loss of ephrin B1 disturbs tissue boundary formation at the developing coronal suture. EFNB1 mainly plays a central part in cell adhesion, angiogenesis, and development of the nervous system.

References:

1. Shi Y, et al. (2021). J Oncol. vol. 4701680. Nov.16.
2. Wallis D, et al. (2008). Am J Med Genet A.146A(15):2008-2012.
3. Arthur A, et al. (2019). Exp Hematol. 69:43-53.