Recombinant Human EGFR protein (Myc & 6*His)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
Myc Tag, His Tag
Activity
not tested
Cat no : Eg0204
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human EGFR protein Leu25 - Ser645 (Accession# P00533) with a Myc tag and a His tag at the C-terminus. |
| GeneID | 1956 |
| Accession | P00533 |
| PredictedSize | 74.2 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Epidermal growth factor receptor (EGFR, also known as ErbB1/HER1) is a cell-surface receptor tyrosine kinase that belongs to the ErbB family of receptors composed of four closely related members: EGFR (ErbB1/HER1), ErbB2 (HER2), ErbB3 (HER3) and ErbB4 (HER4). Under normal physiological conditions, EGFR is expressed in nearly all cell types and tissues, with the exception of hematopoietic lineage cells, and those in bone marrow, spleen, soft tissues, adrenal gland and specific brain tissues, where the EGFR protein is undetectable.
References:
1. Wee, Ping, and Zhixiang Wang. Cancers vol. 9,5 52. 2. Hintzen, Gabriele et al. Frontiers in oncology vol. 12 892212.