Recombinant Human EPHA3 protein (rFc Tag) (HPLC verified)

• Home
• Products
• Fusion Proteins
• AuthentiKine ELISA Kits
• Recombinant Human EPHA3 protein (rFc Tag) (HPLC verified) (Eg2951)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2951

---

Validation Data Gallery

• 

  Purity of Recombinant Human EPHA3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human EPHA3 was greater than 90% as determined by SEC-HPLC.

---

Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human EPHA3 protein Glu21-Gln541 (Accession# P29320-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 2042
• Accession: P29320-1
• PredictedSize: 84.9 kDa
• SDS-PAGE: 85-110 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Ephrin type-A receptor 3 (EPHA3) is a member of the EPH receptor family of tyrosine kinases. These receptors play a crucial role in developmental processes, particularly in the nervous system, and are involved in cell adhesion, migration, and signaling. EPHA3 binds promiscuously membrane-bound ephrin family ligands on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. It has been correlated with various human malignancies, including hematologic disorders, gastric cancer, glioblastoma multiforme, colorectal cancer, etc.

References:

1.Lawrenson ID. et al. (2002). J Cell Sci. 115(Pt 5):1059-1072.
2.London M. et al. (2020). Mol Biol Rep. 47(7):5523-5533.