Recombinant Human EVI2B protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3709

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Validation Data Gallery

• 

  Purity of Recombinant Human EVI2B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human EVI2B protein Lys22-Ser202 (Accession# P34910-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 2124
• Accession: P34910-1
• PredictedSize: 45.7 kDa
• SDS-PAGE: 55-90 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Ecotropic viral integration site 2B (EVI2B), also known as CD361, was first reported to be a common viral integration site in retrovirally-induced murine leukemia. Zjablovskaja et al. showed EVI2B was necessary for granulocytic differentiation and functionality of hematopoietic progenitors.

References:

1. Cawthon R.M., et al. Genomics. 1991;9:446–460.
2. Buchberg A.M., et al. Mol. Cell Biol. 1990;10:4658–4666.
3. Zjablovskaja P., et al. Cell Death Differ. 2017;24:705–716.