Recombinant Human FCRL1 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3713

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Validation Data Gallery

• 

  Purity of Recombinant Human FCRL1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human FCRL1 protein Ala17-Ser307 (Accession# Q9NQ30-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 115350
• Accession: Q96LA6-1
• PredictedSize: 57.9 kDa
• SDS-PAGE: 60-80 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

FCRL1 (Fc receptor–like 1) is a cell-surface membrane protein belonging to FCRL family and is preferentially expressed on B cells. FCRL1 was first identified as an activating receptor capable of enhancing signaling through the BCR. Human FCRL1 consists of three Ig-like domains and two ITAM-like sequences in its cytoplasmic tail.

References:

1. Du X, et al. (2008). Blood. 111(1):338-43.
2. DeLuca JM, et al. (2021). J Immunol. 207(11):2688-2698.