AuthentiKine™ Human FGF19 ELISA Kit
Cat no : KE00751
Synonyms
FGF 19, FGF-19, Fibroblast growth factor 19, UNQ334/PRO533
Validation Data Gallery
Product Information
KE00751 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FGF19 ELISA kit is to be used to detect and quantify protein levels of endogenous human FGF19. The assay recognizes human FGF19. An antibody specific for human FGF19 has been pre-coated onto the microwells. The human FGF19 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FGF19 is added to detect the captured human FGF19 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human FGF19 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9965 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 79%-112% |
| Cell culture supernatant | 83% | 73%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 242.6 | 7.7 | 3.2 |
| 2 | 8 | 59.8 | 2.7 | 4.5 |
| 3 | 8 | 34.6 | 1.4 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 243.9 | 11.6 | 4.7 |
| 2 | 16 | 59.7 | 3.0 | 5.0 |
| 3 | 16 | 32.4 | 2.8 | 8.6 |
Background Information
Fibroblast growth factor (FGF)19 is a hormone that regulate metabolic processes particularly during feeding or starvation, thus ultimately influencing energy production. FGF19 is secreted by the intestines during feeding and negatively regulates bile acid synthesis and secretion. FGF19 is regarded as late-acting hormones because its functions are only used after insulin and glucagon have completed their actions. FGF19 shows extensively functional overlap in terms of improving glucose tolerance, insulin sensitivity, weight loss, and lipid, and energy metabolism, particularly in pathological conditions such as diabetes, obesity, metabolic syndrome, and cardiovascular and renal diseases. Most patients with these metabolic diseases exhibit reduced serum FGF19 levels, which might contribute to its etiology.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FGF 19, FGF-19, Fibroblast growth factor 19, UNQ334/PRO533 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.