Recombinant Human FGFRL1 protein (Myc Tag, His Tag)

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• Recombinant Human FGFRL1 protein (Myc Tag, His Tag) (Eg0185)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg0185

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Validation Data Gallery

• 

  Purity of Recombinant Human FGFRL1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human FGFRL1 protein Ala25-Pro378 (Accession# Q8N441) with a Myc tag and a His tag at the C-terminus.
• GeneID: 53834
• Accession: Q8N441
• PredictedSize: 43.9 kDa
• SDS-PAGE: 60-75 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Fibroblast growth factor receptor-like 1 (FGFRL1) is a member of the FGFR family. It is a type I transmembrane protein which contains three extracellular Ig-like modules, a transmembrane segment, and a short intracellular tail with a peculiar histidine-rich motif. It lacks the intracellular protein tyrosine kinase domain required for signal transduction by transphosphorylation. FGFRL1 binds to FGF ligands and heparin with high affinity. It exerts a negative effect on cell proliferation, but a positive effect on cell adhesion and cell differentiation.

References:

1. M Wiedemann, et al. (2000) Genomics. 69(2):275-9.
2. Beat Trueb, et al. (2003) J Biol Chem. 278(36):33857-65.
3. Beat Trueb. (2011) Cell Mol Life Sci. 68(6):951-64. 
4. Xiaochen Yang, et al. (2016) Int J Mol Med. 38(1):30-8.