Recombinant Human Ferritin heavy chain/FTH1 protein (rFc Tag)

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• Recombinant Human Ferritin heavy chain/FTH1 protein (rFc Tag) (Eg4767)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4767

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Validation Data Gallery

• 

  Purity of Recombinant Human Ferritin heavy chain was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Ferritin heavy chain protein Met1-Ser183 (Accession# P02794) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 2495
• Accession: P02794
• PredictedSize: 47.5 kDa
• SDS-PAGE: 47-60 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo. FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.

References:

1. Mimi C Sammarco, et al. (2008) J Biol Chem. 283(8):4578-87.
2. Maddalena Di Sanzo, et al. (2020) Cells. 9(12):2554.