Recombinant Human Growth Hormone protein (His Tag)

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• Recombinant Human Growth Hormone protein (His Tag) (Eg0383)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0383

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Validation Data Gallery

• 

  Purity of Recombinant Human Growth Hormone was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Growth Hormone protein Phe27-Phe217 (Accession# P01241-1) with a His tag at the N-terminus.
• GeneID: 2688
• Accession: P01241-1
• PredictedSize: 22.9 kDa
• SDS-PAGE: 20-22 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Growth hormone (GH) is a heterogeneous polypeptide consisting of a number of different isoforms and variants. Its secretion from pituitary somatotrophs is pulatile, with low or undetectable basal levels between peaks. GH secretion has a characteristic diurnal pattern with maximal levels recorded during sleep. Growth hormone is a powerful anabolic hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. The actions of GH are mediated by GH receptors (GHR), which are widely distributed among tissues. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.

References:

1. Strobl JS. et al.(1994) Pharmacol Rev. 46(1):1-34.
2. Baumann GP. et al. (2009) Growth Horm IGF Res. 19(4):333-40.
3. Catchpole HR. et al. (1980) JAMA. 243(7):652.
4. Murray RD. et al. (2000) Expert Opin Pharmacother.1(5):975-90