Recombinant Human Glycophorin A protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2336

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Validation Data Gallery

• 

  Purity of Recombinant Human Glycophorin A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Glycophorin A protein Ser20-Glu91 (Accession# ADU25344.1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 2993
• Accession: ADU25344.1
• PredictedSize: 34.2 kDa
• SDS-PAGE: 38-65 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Glycophorin A (GYPA) is a type I single-span membrane protein made with an amino-terminal signal sequence that is cotranslationally cleaved during biosynthesis in the ER, and then traffics to the red cell surface. Glycophorin A carries the M and N antigens of the MNS blood group system. The MN specificity is in the NH2-terminal region, and these antigens require the NH2-terminal O-glycosidic oligosaccharides for activity.

References:

1. Cordat E. et al. (2014). Curr Top Membr. 73:1-67.
2. Toivanen A. et al. (2008). Biochim Biophys Acta. 1780(3):456-466.