Recombinant Human Granzyme A protein (mFc Tag) (HPLC verified)

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• Recombinant Human Granzyme A protein (mFc Tag) (HPLC verified) (Eg3925)

Species

Human

Purity

>90 %, SDS-PAGE
>90%, SEC-HPLC

Tag

mFc Tag

Activity

not tested

Cat no : Eg3925

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Validation Data Gallery

• 

  Purity of Recombinant Human Granzyme A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human Granzyme A was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90%, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Granzyme A protein Glu27-Val262 (Accession# P12544-1) with a mouse IgG Fc tag at the C-terminus.
• GeneID: 3001
• Accession: P12544-1
• PredictedSize: 52.7 kDa
• SDS-PAGE: 50-60 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Granzyme A (GzmA) is a serine protease primarily expressed by cytotoxic T lymphocytes and natural killer (NK) cells, playing a critical role in immune-mediated cytotoxicity. It is involved in the elimination of target cells, such as virus-infected cells or tumor cells, by inducing apoptosis. GzmA is associated with various diseases, particularly those involving immune responses and inflammation. It plays a significant role in the pathogenesis of sepsis, where its inhibition reduces inflammation and increases survival.

References:

1.Zhou Z. et al. (2020). Science. 368(6494):eaaz7548.
2.Hay ZLZ. et al. (2022). Int J Mol Sci. 23(3):1833. 
3.Garzón-Tituaña M. et al. (2021). Theranostics. 11(8):3781-3795.