AuthentiKine™ Human IFN-gamma ELISA Kit

Sensitivity

0.4 pg/mL

Range

7.8-500 pg/mL

Reacted Species

Human

Cat no : KE00146

Synonyms

IFG, IFI, IFN gamma, IFNG, IFN-gamma, Immune interferon, Interferon gamma, interferon, gamma



Product Information

KE00146 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN-gamma ELISA kit is to be used to detect and quantify protein levels of endogenous IFN-gamma. The assay recognizes human IFN-gamma. An antibody specific for IFN-gamma has been pre-coated onto the microwells. The IFN-gamma protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IFN-gamma is added to detect the captured IFN-gamma protein. For signal development, horseradish peroxidase (HRP)-conjugated is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human IFN-gamma ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture superntants
Assay type Sandwich
Sensitivity 0.4 pg/mL
Range 7.8-500 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma96%84%-107%
Cell culture superntants95%89%-107%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120535.917.53.3
22099.82.52.5
32022.81.25.3

InterAssay

Samplenmean ( pg/mL)SDCV%
124517.422.64.4
224115.66.96.0
32428.52.89.8

Background Information

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms IFG, IFI, IFN gamma, IFNG, IFN-gamma, Immune interferon, Interferon gamma, interferon, gamma