Recombinant Human IFNA21 protein (rFc Tag)(HPLC verified)

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• Recombinant Human IFNA21 protein (rFc Tag)(HPLC verified) (Eg5101)

Species

Human

Purity

>90 %, SDS-PAGE
>90%, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg5101

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Validation Data Gallery

• 

  Purity of Recombinant Human IFNA21 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human IFNA21 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90%, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human IFNA21 protein Cys24-Glu189 (Accession# P01568) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 3452
• Accession: P01568
• PredictedSize: 45.3 kDa
• SDS-PAGE: 45-52 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interferons (IFN) are a family of cytokines with potent anti-viral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors. Individual IFNA subtypes are known to display unique efficacies to viral protection, with IFNA21 displaying intermediate activity inducing interferon stimulating genes.

References:

1. Pestka S. et al. (1987). Annu. Rev. Biochem. 56:727.
2. Moll H.P. et al. (2011). Cytokine. 53:52.