Recombinant Human IFNA4 protein (rFc Tag)(HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg4962

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Validation Data Gallery

• 

  Purity of Recombinant Human IFNA4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human IFNA4 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human IFNA4 protein Cys24-Asp189 (Accession# P05014) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 3441
• Accession: P05014
• PredictedSize: 46.6 kDa
• SDS-PAGE: 45-52 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interferon Alpha 4 (IFNA4) belongs to the alpha/beta interferon family. IFNA4 is predicted to enable cytokine activity and type I interferon receptor binding activity, and might be involved in several processes, including B cell activation.

References:

1. Henco K. et al. (1985). J Mol Biol. 185(2):227-60.
2. Hussain M. et al. (1997). J Interferon Cytokine Res. 17(9):559-66.