Recombinant Human IGLV3-21 protein (rFc Tag)(HPLC verified)

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• Recombinant Human IGLV3-21 protein (rFc Tag)(HPLC verified) (Eg2591)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2591

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Validation Data Gallery

• 

  Purity of Recombinant Human IGLV3-21 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human IGLV3-21 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human IGLV3-21 protein Val18-Thr117 (Accession# P80748) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 28796
• Accession: P80748
• PredictedSize: 36.7 kDa
• SDS-PAGE: 40-62 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The light chain IgLV3-21 (previously named “VL2-14”) represents the most abundant lambda light chain in CLL (~21%) and is the third most abundant light chain (~7%, after IgKV3-20 and IgKV1-39) if both lambda and kappa light chains are considered.

References:

1. Stamatopoulos K, et al. (2005) Blood. 106(10):3575-83.