Recombinant Human IL-1 Beta protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0286

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Validation Data Gallery

• 

  Purity of Recombinant Human IL-1 Beta was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human IL-1 Beta protein Ala117-Ser269 (Accession# P01584 ) with a His tag at the N-terminus.
• GeneID: 3553
• Accession: P01584
• PredictedSize: 18.2 kDa
• SDS-PAGE: 19-20 kDa, reducing (R) conditions
• Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.

References:

1. Takada YK. et al. (2017). J Biol Chem. 292: 20067-20075.
2. LaRock DL. et al. (2020). Infect Immun. 88: e00356-20.
3. Tian XX. et al. (2021). Int Endod J. 54: 916-925.
4. Jourdan T. et al. (2013). Nat Med. 19: 1132-1140.